| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070781 |
| Chromosome: | chromosome 16 |
| Location: | 1234594 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650950 | (1 of 1) PF01931 - Protein of unknown function DUF84 (NTPase_I-T) | 5'UTR | |
| Cre16.g651000 | RFA3,RPA70B | (1 of 1) PTHR23273:SF4 - REPLICATION PROTEIN A 70 KDA DNA-BINDING SUBUNIT; Replication protein A, 70 kDa DNA-binding subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAACCTCTTTTCGACAGTGTAACAAGTCCAGGGCTGCCGTGCAAAGCT |
| Internal bar code: | CATTTAGAGACCCCTGTAAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2292 |
| LEAP-Seq percent confirming: | 38.9831 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCGATTGCTGCTGAAGGT |
| Suggested primer 2: | GGGAACACCAGCTTGCTTTG |