Insertion junction: CLIP2.070785_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.070785
Chromosome:chromosome 9
Location:6538826
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g415950 PRPL6,uL6m,MRPL6 (1 of 1) K02933 - large subunit ribosomal protein L6 (RP-L6, MRPL6, rplF); Mitochondrial/chloroplast ribosomal protein L10 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCGCAGCCGCTGAGGGCTAGGGGACGAGATGGAGCCGCGTCTCCGCTT
Internal bar code:CTGACGGCTGTTACAAGGCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1753
LEAP-Seq percent confirming:93.3333
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:ACACTGCCCTCCCCTGATAT
Suggested primer 2:TGTTGGTGCTGTGGAAACCT