Insertion junction: CLIP2.070792_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.070792
Chromosome:chromosome 1
Location:6066614
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g042850 (1 of 2) K17278 - membrane-associated progesterone receptor component (PGRMC1_2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCCACCCCCTTCCACCTTGCCTTCCACACGGCGCTTTCCTGCCCACAA
Internal bar code:ATCGCTATTATCCGGCGTAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:463
LEAP-Seq percent confirming:4.7619
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CACGCGGTATTGCATGACTG
Suggested primer 2:CTATCGGGAGGGGCGTTTTT