| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070798 |
| Chromosome: | chromosome 2 |
| Location: | 41693 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g073200 | THD1 | putative threonine dehydratase and component psaA trans-splicing sub complex II; (1 of 1) K01754 - threonine dehydratase (E4.3.1.19, ilvA, tdcB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGGGGTCGTATCTTGTTTTTGGGGTAATGTCTAGCTCCAGACAAAAA |
| Internal bar code: | TTGGGTGTTCAGGCCGGCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4873 |
| LEAP-Seq percent confirming: | 96.7391 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGTTGACAACTGTGCTG |
| Suggested primer 2: | CGAATTGCACCACTTTGGCA |