| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070807 |
| Chromosome: | chromosome 1 |
| Location: | 5361306 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037850 | BCC2,BXP2, BCCP2 | Acetyl-CoA biotin carboxyl carrier; (1 of 1) IPR000089//IPR011053 - Biotin/lipoyl attachment // Single hybrid motif | 3'UTR |
| lncRNA_TCONS_00014348 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGAGCCGCTGGTGGCAGCGGGACCGCCCTGGGTACGTGTGTGCATATG |
| Internal bar code: | GGCATGCGCGGGATGTTGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1794 |
| LEAP-Seq percent confirming: | 60.7143 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACAAGCGACCAGGAAGTC |
| Suggested primer 2: | GCTATTGCCTCGAATGCAGC |