Insertion junction: CLIP2.070811_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.070811
Chromosome:chromosome 13
Location:702201
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g566450 ROC104 (1 of 66) 2.7.12.1 - Dual-specificity kinase; Rhythm of Chloroplast bioluminescence 104 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATAACTTAAGCTCAGCGCAACGAGCGCTTGCGTATACAATTTCTTCGT
Internal bar code:TATTCGAGCTGCCTGATCTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1502
LEAP-Seq percent confirming:97.0588
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCTTGCCAGAGTCCTGTT
Suggested primer 2:TAGCACAGCACAGTAGCACC