Insertion junction: CLIP2.070826_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.070826
Chromosome:chromosome 16
Location:745750
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g690900 RBL8 rhomboid-like protein; (1 of 4) PTHR22936//PTHR22936:SF32 - RHOMBOID-RELATED // SUBFAMILY NOT NAMED 5'UTR
Cre16.g690950 (1 of 54) IPR011047 - Quinonprotein alcohol dehydrogenase-like superfamily 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCACGTGTCGCAGCGCAAATGTGTGTGGGTGCACCGCTTTGCACCATCT
Internal bar code:ATACCATCATCGTTTATAACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1468
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGTGTGTGCGTGTCAAG
Suggested primer 2:TGCGCAGGCTTGAAGTAAGA