Insertion junction: CLIP2.070831_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.070831
Chromosome:chromosome 17
Location:4862899
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre17.g734961 (1 of 1) PTHR15239:SF6 - NUCLEAR EXPORT MEDIATOR FACTOR NEMF 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTCCTGGGCCCAGCATCTGGCGCCAACACACACAACTCCGCTCCGCCG
Internal bar code:CAAGAGTTCTTAATCGCCTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:106
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TACAAGGTCAAGGTCACGCC
Suggested primer 2:TGAGGTGACGAAGACAACGG