| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070842 |
| Chromosome: | chromosome 12 |
| Location: | 5787775 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532800 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCTACGCGGCCGCCAAGACGCGCACAGTCAGTGTGGCGGCGGCGGAG |
| Internal bar code: | GATGGGGCATGCCATGTCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 800 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAATCCTGAAGGGGTGGGG |
| Suggested primer 2: | ACCTCCTCCTTCCCTCCATC |