| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070860 |
| Chromosome: | chromosome 17 |
| Location: | 222789 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697334 | (1 of 10) PF13519 - von Willebrand factor type A domain (VWA_2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGCCAGAGGGGTTGTCATGAGCACCCGAGCCCAATGCATCAGGTTTG |
| Internal bar code: | AACTGATTAGTTGCATTCACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 902 |
| LEAP-Seq percent confirming: | 69.3548 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCTCCGCTTTTCCTCCT |
| Suggested primer 2: | TTCACGGGTACAGGGAAAGC |