| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070860 |
| Chromosome: | chromosome 17 |
| Location: | 222789 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697334 | (1 of 10) PF13519 - von Willebrand factor type A domain (VWA_2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCCACAACACACGAAGCAAATCCTAATTGATATTGTGAGTGCTTCTT |
| Internal bar code: | GCACTTACTAGGACATCTGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1138 |
| LEAP-Seq percent confirming: | 86.8421 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCACGGGTACAGGGAAAGC |
| Suggested primer 2: | GCTTCTCCGCTTTTCCTCCT |