Insertion junction: CLIP2.070864_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.070864
Chromosome:chromosome 6
Location:7282395
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g299100 3'UTR
Cre06.g299150 PHC9,PHC26 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 26 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGATCAACCGCATCGCTGGCAGTGGCAGGTGTGCCCTCAGGCGCATGC
Internal bar code:ACGGTGGTTAGGAGCCAAAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:547
LEAP-Seq percent confirming:2.5
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:AGAAGGCGAAGAAGAAGGCC
Suggested primer 2:TCTGTCCAAAATCCGCCGAA