| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070870 |
| Chromosome: | chromosome 13 |
| Location: | 3476301 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587450 | FCL2 | Predicted 5-formyltetrahydrofolate cycloligase; (1 of 1) PTHR13017:SF0 - METHENYLTETRAHYDROFOLATE SYNTHASE DOMAIN-CONTAINING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCAATACCAAACAATAAAGCATTCAAGCATTCCCAGCAAAATGACCA |
| Internal bar code: | CACAGTAAGGAATTCACTGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2781 |
| LEAP-Seq percent confirming: | 98.1818 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGATCCGGATCCTGCAG |
| Suggested primer 2: | CAAGGTTCACTGGCTTTGGC |