| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070878 |
| Chromosome: | chromosome 2 |
| Location: | 3998124 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102750 | (1 of 2) PTHR31646:SF1 - ALPHA-1,2-MANNOSYLTRANSFERASE MNN2 | 3'UTR | |
| Cre02.g102776 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGACTTTCGACGTATGGAAGGATGGCCCCGGCCGGGCCGACCATGCAT |
| Internal bar code: | TCATTTTGTTATCTTGGGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4693 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATTAAGCTCGCATGGTGC |
| Suggested primer 2: | ACTTCTACTGGTGTGTGCGG |