| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070879 |
| Chromosome: | chromosome 6 |
| Location: | 8116875 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305550 | 5'UTR | ||
| Cre06.g305600 | ADH9 | Alcohol dehydrogenase; (1 of 1) 1.1.1.329 - 2-deoxy-scyllo-inosamine dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCTTTACAAAAAGGGAGCTCGGGTACATACACACGTCAATCAACAGA |
| Internal bar code: | CGTTGGCTCGCATGGATAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2150 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGTCTATGGGTTTGGTCA |
| Suggested primer 2: | GGGAGGTCCAGATGGGTAGT |