| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070882 |
| Chromosome: | chromosome 12 |
| Location: | 5352174 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528900 | RBG1 | 60S ribosome subunit biogenesis protein; (1 of 1) K07565 - 60S ribosome subunit biogenesis protein NIP7 (NIP7) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATTATCTGTTTCAGGTGCAGTACCAACACATTGGCTCTCCGTGTGTG |
| Internal bar code: | GGTCACGAGCTGTATGAGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4850 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 100 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 100 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGCCCGTGCGTTAAAAA |
| Suggested primer 2: | CGTGGAATGCACGCAATAGG |