| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070889 |
| Chromosome: | chromosome 7 |
| Location: | 5391285 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350450 | (1 of 2) PTHR22763:SF71 - PROTEIN Y105C5B.11, ISOFORM B | outside_mRNA | |
| Cre07.g350451 | (1 of 2) IPR001680//IPR017986//IPR020472//IPR027417 - WD40 repeat // WD40-repeat-containing domain // G-protein beta WD-40 repeat // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCACCCTACCTCGCTTGTCACTCATGTTCACCGTCGCAGACATAGCGC |
| Internal bar code: | TGCATAAATTAGGTTCCCGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4599 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAAGCGGTAGTTGGTGT |
| Suggested primer 2: | TAATCTGGTCGCTCGGATGC |