| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070907 |
| Chromosome: | chromosome 7 |
| Location: | 5573947 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351900 | (1 of 7) 2.7.10.2//2.7.12.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Dual-specificity kinase | 5'UTR | |
| Cre07.g351950 | ZRT1,CrZIP1 | (1 of 6) K14709 - solute carrier family 39 (zinc transporter), member 1/2/3 (SLC39A1_2_3, ZIP1_2_3); Zinc-nutrition responsive transporter | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTATTTCTCGACTTCCGCTGCTAAAACCCACCCGGCCCAGCTGTCAGC |
| Internal bar code: | GGGAAGTATCAGTTATAATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1764 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGAAGCATGGATGGGGG |
| Suggested primer 2: | GCTGGTATTGCGCAAGATGG |