| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.070921 |
| Chromosome: | chromosome 17 |
| Location: | 96168 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g696600 | FMR1 | (1 of 1) PTHR11632:SF6 - PROTEIN F48E8.3; Fumarate reductase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCTGCCCAAACCTGAACCCGCCAAAACCTGAACCCGCCTAACCCTGTA |
| Internal bar code: | TTTAGCGCGCAAGCGCGAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1745 |
| LEAP-Seq percent confirming: | 96.4912 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAGAGGGCTAGCGCTGAG |
| Suggested primer 2: | CGCCTCTTCACCTTGGTCAT |