| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.070923 |
| Chromosome: | chromosome 12 |
| Location: | 622990 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493500 | SOX1 | (1 of 1) PTHR10961 - PEROXISOMAL SARCOSINE OXIDASE; Sarcosine oxidase | 3'UTR |
| Cre12.g493550 | THK3 | Thymidine kinase; (1 of 1) PTHR11652//PTHR11652:SF1 - 30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER // 28S RIBOSOMAL PROTEIN S12, MITOCHONDRIAL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTATTCACATCGTAGCTCGTTACAGACTCCGCCCATATTAAGACATAC |
| Internal bar code: | CGCCTCTTCCGATGGATCGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 668 |
| LEAP-Seq percent confirming: | 90.6977 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAGCACATAGGCCAGGGA |
| Suggested primer 2: | AGCCATGCCAGTGTTACCTC |