| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070969 |
| Chromosome: | chromosome 12 |
| Location: | 5157704 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526800 | GND1 | (1 of 1) 1.1.1.44 - Phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating) / Phosphogluconic acid dehydrogenase; 6-phosphogluconate dehydrogenase, decarboxylating | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCCTGCCTAGTGCACCTCCTTCACGTGCTCCATGCGAGTTGCCCTGC |
| Internal bar code: | CGGCTCAGGAGTACCGGGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 41.6667 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCGTTCCCAATAAGCATG |
| Suggested primer 2: | AGGAGAGCAGTGTGATGTGC |