| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070994 |
| Chromosome: | chromosome 17 |
| Location: | 2432193 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g714800 | ISG6,ISG-C3 | (1 of 5) PTHR16631:SF9 - GLUCAN 1,3-BETA-GLUCOSIDASE; Hydroxyproline-rich cell wall protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAAAACAGCTGGGCGTGTAACCCCTTGCGGGCTTGGTGAGGCGCTCTC |
| Internal bar code: | GGGCGGAGTGGTGCCATCTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5234 |
| LEAP-Seq percent confirming: | 86.8687 |
| LEAP-Seq n confirming: | 86 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 99 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCAGCTAGATACAGCCCA |
| Suggested primer 2: | CCCACTGACTTCGTCACGAA |