| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070996 |
| Chromosome: | chromosome 3 |
| Location: | 2757628 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g161450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAAACCGAACTCTCCTCCTGGACAAGCGCGCTAAACCCCTTGCAATT |
| Internal bar code: | ATAGACTCTAATGCAACGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5972 |
| LEAP-Seq percent confirming: | 99.0566 |
| LEAP-Seq n confirming: | 105 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 106 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTAGGACTGGGTTTGCC |
| Suggested primer 2: | ATTACGCTTGGAATGCACGC |