| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071009 |
| Chromosome: | chromosome 9 |
| Location: | 6697666 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g416900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCTGTGCCCCCGCAACCACACTGATCGCGCACATCAGTCACGGACTA |
| Internal bar code: | TTGTCCAATTGTAACTTAATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1571 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTTGTCAACGCAGGCAGG |
| Suggested primer 2: | GGGCTGCCTGGTATTGTTCT |