| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.071017 |
| Chromosome: | chromosome 8 |
| Location: | 4131813 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383450 | FIST N domain protein; (1 of 3) PF08495 - FIST N domain (FIST) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGCGGAGGTCAGCCGAACAGCCCAGCCAGCCCAGCCCAGCCCAGCCC |
| Internal bar code: | ATCGCTCGATCAGGTAATTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 141 |
| LEAP-Seq percent confirming: | 41.6667 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTGTTTGTGTCTGCGGC |
| Suggested primer 2: | GTAGTCCAGCCCCTCCAGTA |