| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071019 |
| Chromosome: | chromosome 3 |
| Location: | 1055545 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g148250 | CGL58 | (1 of 1) PTHR15856//PTHR15856:SF25 - PHD FINGER PROTEIN 20-RELATED // SUBFAMILY NOT NAMED; Conserved in the Green Lineage | 3'UTR |
| Cre03.g148300 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAAGCACCCCGCCCCGCCCTGCACGCCGCGCTGCCCCTGACTAGCAT |
| Internal bar code: | AGATTGCAACGTTTTCCTTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2099 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCCCGCACTCATCATCG |
| Suggested primer 2: | ATGAGGCAGCAGTTCCTGAC |