| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071028 |
| Chromosome: | chromosome 1 |
| Location: | 6264157 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044450 | (1 of 15) PF01694 - Rhomboid family (Rhomboid) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCTGAGGCAGGCCTGAGTCCCCAGGAAAAGGCGGGGCCGTGAAGGGG |
| Internal bar code: | TTTCGTATAGGGTAAAACAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2682 |
| LEAP-Seq percent confirming: | 97.2603 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGACGAAGCAAAGGAGG |
| Suggested primer 2: | GCTTGGGAAGGAGCATGGAT |