| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071033 |
| Chromosome: | chromosome 16 |
| Location: | 6107411 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801985 | (1 of 1) K10740 - replication factor A3 (RPA3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAAGCGAGAGTTTTGGAGTGGTTCTGCAATGAGCGTGAATGATGCACC |
| Internal bar code: | AAGTCGACGATTTACAATGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2503 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCAACGCTGCTTCCGATG |
| Suggested primer 2: | CAGTGTGCGTGTGTCTAGGT |