Insertion junction: CLIP2.071037_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.071037
Chromosome:chromosome 5
Location:3479121
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre05.g233450 ELG30 (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 30 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTGTCAGGCACGCTGTCACTGCCAGCCGCCGCGCCACCCGCAGACGCC
Internal bar code:TTACACAATCGCGGATGAGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:90
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCCTGCCTGTAACATGCA
Suggested primer 2:CTGTGCCATCTTTGCAACCC