| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071040 |
| Chromosome: | chromosome 7 |
| Location: | 2511888 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329650 | (1 of 1) PTHR23004//PTHR23004:SF7 - DOUBLECORTIN DOMAIN CONTAINING 2 // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCACCTTGCATGCGGTAGCGCTCTGCGCGTCGTGCTGCGCGGCGCCGC |
| Internal bar code: | AGCACCCCCGCGATTGTGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1292 |
| LEAP-Seq percent confirming: | 79.1667 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCGGCTTACATGTTGCCT |
| Suggested primer 2: | ATCATCACGTACCGATGGCC |