| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071049 |
| Chromosome: | chromosome 13 |
| Location: | 3936570 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801566 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGTACCGCGGACTCCATACCGGAATACTGACTCGGTTCCCCGGGCAGG |
| Internal bar code: | CCTATTGTTCTTGACGAAAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5712 |
| LEAP-Seq percent confirming: | 21.4286 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTGACGCCAGCATAT |
| Suggested primer 2: | AAAGTGTGGAGAGGGCATCG |