Insertion junction: CLIP2.071056_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.071056
Chromosome:chromosome 7
Location:5657571
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g352600 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATTCGATTGCGGGCTGCTCTTGGGATAATCGAGTGCTATGTGGCACAG
Internal bar code:AAAAAATATGGGAATCGATACG

Confirmation - LEAP-Seq

LEAP-Seq distance:82
LEAP-Seq percent confirming:7.69231
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CAGAGCGGATTTGATGCTGC
Suggested primer 2:CAGTCCCCTGCTGTACTGTG