| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071060 |
| Chromosome: | chromosome 11 |
| Location: | 1348104 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467710 | VSP1 | Hydroxyproline-rich cell wall glycoprotein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAAGGCGAGTCCTCAAGGTCAGGCGAAGGAGACGGCGAGTCTTCCGC |
| Internal bar code: | CTTATGCATCCCTAGGCCTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 389 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTACAGACACGCCTTGGT |
| Suggested primer 2: | CTGGTTAGGCACATCCAGCA |