| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071062 |
| Chromosome: | chromosome 7 |
| Location: | 2423013 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328900 | CDPK3,CDPK9 | (1 of 6) PTHR24349//PTHR24349:SF23 - SERINE/THREONINE-PROTEIN KINASE // MAP KINASE-ACTIVATED PROTEIN KINASE 5; Calcium/calmodulin-dependent protein kinase | outside_mRNA |
| Cre07.g328950 | (1 of 1) PTHR12277:SF51 - ALPHA/BETA HYDROLASE FOLD-1 DOMAIN-CONTAINING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGCGTCATGTACGTGTGCTCGCGGCGGGGCACCAGTCACAAGTTGGT |
| Internal bar code: | AGGCTGGGGATTTCGTCGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3117 |
| LEAP-Seq percent confirming: | 84.8485 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCAGTGTTCACCAAACGC |
| Suggested primer 2: | CGCAATGTGGTGTAGTCCCT |