| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.071072 |
| Chromosome: | chromosome 9 |
| Location: | 2074330 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396300 | PPX1,PPOX | (1 of 1) 1.3.3.4 - Protoporphyrinogen oxidase / Protoporphyrinogenase; Protoporphyrinogen oxidase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACACTGAGGGACAGTGCCCCCAAACCGCCCCGCGTGTGCGCCAACTCA |
| Internal bar code: | TAGAGGTACTCCTTAGGTTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 801 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGACCTATTTCGTTGGGC |
| Suggested primer 2: | ACGTCCCAAGGCATGCAATA |