| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.071083 |
| Chromosome: | plastome |
| Location: | 91026 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802301 | ycf3,ChreCp037,2717008,pafI | (1 of 1) PTHR26312:SF81 - PHOTOSYSTEM I ASSEMBLY PROTEIN YCF3; photosystem I assembly factor I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAAATTATCATTTCTTTGCGTTCTTGGCATTAAAAATAAAATCAGCGA |
| Internal bar code: | CATTGCTGTTGGTCTCCTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTCATTGCCGTCCTTTGG |
| Suggested primer 2: | ACTGAGGGGCTGCAATTTGT |