| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.071087 |
| Chromosome: | chromosome 3 |
| Location: | 5595521 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185600 | OGD3 | (1 of 3) 2.3.1.61 - Dihydrolipoyllysine-residue succinyltransferase / Succinyl-CoA:dihydrolipoate S-succinyltransferase; Dihydrolipoamide succinyltransferase | 5'UTR |
| Cre03.g185650 | CaM-IP4,FAP251 | (1 of 1) PTHR13720//PTHR13720:SF13 - WD-40 REPEAT PROTEIN // WD REPEAT-CONTAINING PROTEIN 66; Flagellar Associated Protein 251 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAAGGTTTTGGTGCCGGGTTTTGGACACCCATTCAGAACGTAGCTCAT |
| Internal bar code: | GCGGTATTAATAGTATCAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3889 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACATCGGGCATACGCAG |
| Suggested primer 2: | GAGTACGTGGTGCTGTGTGA |