| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071125 |
| Chromosome: | chromosome 2 |
| Location: | 6989219 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389467 | OPR8 | (1 of 7) IPR014721 - Ribosomal protein S5 domain 2-type fold, subgroup; OctotricoPeptide Repeat protein 8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAGCGCATACCACTCAGTCCCCTTGTCCCCACAGGGGGGGGTGCAGCC |
| Internal bar code: | AAAGGGTAGGGCTATTTTTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1825 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAATCGCTACCGGTGATA |
| Suggested primer 2: | CGAGTACTTGGGTGAAGGGG |