Insertion junction: CLIP2.071127_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.071127
Chromosome:chromosome 10
Location:5300254
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g456500 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACATTTCGTTTGATGATAGATGTGGTAGAGGTACATGCTTGCTGTGTGT
Internal bar code:CTGACAAAGCGGCTTAAGAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1262
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:ATTACTCTCTGACGCGAGCG
Suggested primer 2:GAGAGACACGGTTGAGCCAA