Insertion junction: CLIP2.071135_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.071135
Chromosome:chromosome 5
Location:393504
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre05.g243600 GOX2 Glyoxal or galactose oxidase pseudogene; (1 of 1) IPR013783//IPR014756//IPR015202 - Immunoglobulin-like fold // Immunoglobulin E-set // Domain of unknown function DUF1929 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGATCACACCACCTACCGCGCCGTCCAGCATCTAGGTCCGAAGCCTACG
Internal bar code:CTAGCGTACTTCCCGGTAATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:70
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTGGAGATCCTGTCCAAC
Suggested primer 2:GAATTACGCCAAGCCTGCTG