| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071146 |
| Chromosome: | chromosome 4 |
| Location: | 3095156 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225650 | (1 of 1) PF00031 - Cystatin domain (Cystatin) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATTTGCTGCCGCAATGCACTTGTGTAGACCTACGTGGACTGCGGAGTG |
| Internal bar code: | GAGAACTGGTTTTTTCTAGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2631 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGGTAAGGTACTCGCCG |
| Suggested primer 2: | AGAAAGCCGAACCGCAAGTA |