| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071172 |
| Chromosome: | chromosome 7 |
| Location: | 4569131 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g800837 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAGAAAGACGTATCTGCGTCATGCCATGTGTGTCTGTGTGTTTCCGG |
| Internal bar code: | CGTGGGTCATCTTTTATAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 995 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGGGCTGTCCTTTACAA |
| Suggested primer 2: | AATTACTCTTTGGGCCCGGG |