Insertion junction: CLIP2.071176_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.071176
Chromosome:chromosome 17
Location:571565
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g699650 DIV137 (1 of 3) IPR001441 - Decaprenyl diphosphate synthase-like; Hypothetical protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGAGGTACTTATTGGCGTGCCGGCTGCGCGGCTTACGAGGCTGCGGGGT
Internal bar code:AAAGTTAGAAAATGACTAGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:443
LEAP-Seq percent confirming:1.81818
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:54
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCGGTCTCAAGTGTCGAA
Suggested primer 2:CTTTGGGAGCTAGGTTGGGG