| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071187 |
| Chromosome: | chromosome 3 |
| Location: | 5074516 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180100 | UNG2 | Uracil DNA glycosylase; (1 of 1) 3.2.2.29 - Thymine-DNA glycosylase / Uracil/thymine DNA glycosylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCAAAGGCTGGGGGAGGCAGCCGAAGCAAAACGGCCAAATCCGACTC |
| Internal bar code: | CAAATGCTTCATATGCGAACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 76 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCAAACACCTCGCTCTT |
| Suggested primer 2: | ACCAAAATGGCGGAGGGTAG |