| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071190 |
| Chromosome: | chromosome 13 |
| Location: | 983985 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g568300 | 3'UTR | ||
| Cre13.g568350 | BLZ18 | bZIP transcription factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCTGCGCATGAGGCTGGCGCCAGGGGTGGCGCAAGTCCCGCGGTACT |
| Internal bar code: | GAAACCGCCAGAATCGGAGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1560 |
| LEAP-Seq percent confirming: | 89.6552 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGATGGGTATGAGTGGAG |
| Suggested primer 2: | ACCTTGATGCAGCGACAAGA |