| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071199 |
| Chromosome: | chromosome 7 |
| Location: | 892549 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318702 | (1 of 1) K11806 - WD repeat and SOF domain-containing protein 1 (WDSOF1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCATGCCACAAAACCTCCCGGACCCCCCGACCGACCGACCGACCGAC |
| Internal bar code: | AAGTCCCCCAGCGGTTTCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2670 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTTTCTGGGTGCAAAGT |
| Suggested primer 2: | AGAGCTGGGGGAGTAAGAGG |