| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071213 |
| Chromosome: | plastome |
| Location: | 123138 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802313 | rbcL,ChreCp049,2717040 | (1 of 1) K01601 - ribulose-bisphosphate carboxylase large chain (rbcL); RuBisCO large subunit | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAATGGTTCCACAAACAGAAACTAAAGCAGGTGCTGGATTCAAAGCCG |
| Internal bar code: | GTGACATCCAATGGGGTGAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2848 |
| LEAP-Seq percent confirming: | 86.0465 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAACGCATGAATGGTTGT |
| Suggested primer 2: | TTCTGGAGTACGCATTGCCA |