| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071229 |
| Chromosome: | chromosome 12 |
| Location: | 2722922 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504750 | HTA16,HTA43 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAAACACCGAGTTTTTTTGGGGTGCAACTTGGGGTTGCAACAGGGCAC |
| Internal bar code: | CCCTGTGTGTCAACGTCCTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 18.1818 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGGTTGTTTGACCCAAC |
| Suggested primer 2: | CAAACCCAACACTCAGCAGC |