| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071232 |
| Chromosome: | chromosome 2 |
| Location: | 6665162 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390100 | SMM34 | (1 of 1) 2.1.1.103 - Phosphoethanolamine N-methyltransferase; S-adenosyl-L-methionine-dependent methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATGGCCCCACGCTTGCAGTTGTCCTGCTGCTTGCCCTGACCGCTAAAT |
| Internal bar code: | TTGTAAAATTACTCGTTTTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1502 |
| LEAP-Seq percent confirming: | 82.716 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGAGGTCGAACGGATAG |
| Suggested primer 2: | AGGTTACCGGTATGGGAGCT |