| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071234 |
| Chromosome: | chromosome 10 |
| Location: | 3941367 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446800 | (1 of 3) IPR016087 - Chalcone isomerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGAGGCCTAGTGAAAGGCCCTCCGCCATCTTACCTTGCTATTAATCC |
| Internal bar code: | GGGAACTTAAAAGATCCGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3402 |
| LEAP-Seq percent confirming: | 63.4146 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGGCATATGAGCTGTTTG |
| Suggested primer 2: | GCAAAACCGCAAAAGCACAC |